Promoting sustainable aquaculture and biodiversity conservation: The role of mealworm (Tenebrio molitor) in bone mineralization and amino acid composition of Labeo rohita fingerlings
DOI:
https://doi.org/10.5281/zenodo.20058675Keywords:
Insect protein, Mealworm, Labeo rohita, bone mineralization, amino acid composition, sustainable aquaculture, fishmeal replacementAbstract
Aquaculture is a rapidly growing industry that faces the challenge of providing sustainable, cost-effective, and nutritious feed to meet the increasing global demand for fish protein. Traditionally, fishmeal has been the primary source of protein in aquafeeds; however, concerns about its environmental impact and price volatility have led to the exploration of alternative protein sources. This study investigates the potential of mealworm (Tenebrio molitor) meal as a sustainable protein source for Labeo rohita fingerlings, with a focus on its effects on bone mineralization and amino acid composition. The research was conducted under semi-controlled laboratory conditions, with Labeo rohita fingerlings fed diets containing varying levels of mealworm meal (0%, 25%, 50%, 75%, and 100%) as a replacement for fishmeal. Bone mineralization was assessed by measuring calcium, iron, and phosphorus content, while amino acid composition was analysed using high-performance liquid chromatography. Results demonstrated that the inclusion of mealworm meal positively influenced bone mineralization, with moderate inclusion (50% and 75%) showing the most significant effects on calcium, iron, and phosphorus levels. The amino acid profile also improved, with increased concentrations of essential amino acids, particularly lysine, methionine, and threonine, at the 50% mealworm inclusion level. This study highlights the potential of mealworm meal as an alternative protein source in aquafeeds, offering benefits not only for fish health and growth but also for sustainability in aquaculture. The results suggest that mealworm meal could replace fishmeal in aquafeeds without compromising the nutritional value of Labeo rohita. Future research should focus on optimizing mealworm inclusion levels, assessing the long-term impacts on fish health, and exploring its economic feasibility for large-scale aquaculture.
References
Akpoilih, B. U., Omitoyin, B. O., & Ajani, E. K. (2017). Phosphorus utilization in juvenile Clarias gariepinus fed phytase-supplemented diets based on soya bean (oil-extracted) and full-fat (roasted): A comparison. Journal of Applied Aquaculture, 29(2), 126-151.
Auzins, A., Leimane, I., Reissaar, R., Brobakk, J., Sakelaite, I., Grivins, M., & Zihare, L. (2024). Assessing the socio-economic benefits and costs of insect meal as a fishmeal substitute in livestock and aquaculture. Animals, 14(10), 1461.
Boykin, K., Bitter, A., & Mitchell, M. A. (2021). Using a Commercial Gut Loading Diet to Create a Positive Calcium to Phosphorus Ratio in Mealworms (Tenebrio molitor). Journal of Herpetological Medicine and Surgery, 31(4), 302-306.
Colombo, C., Palumbo, G., He, J.-Z., Pinton, R., & Cesco, S. (2014). Review on iron availability in soil: interaction of Fe minerals, plants, and microbes. Journal of soils and sediments, 14, 538-548.
Debbarma, S., Deb, S., Yadav, N. K., Kashyap, S., Debbarma, P., Chrisolite, B., Yirang, Y., Meena, D. K., Singh, S. K., & Choudhury, T. G. (2025). Waste not, want not: unlocking the innovative potential of organic and eco-friendly insect and algal resources for future aquaculture. Aquaculture International, 33(2), 158.
Gutasi, A. (2021). Benefit and drawbacks of fish meal substitution in aquaculture diets.
Habte-Tsion, H.-M., Hawkyard, M., Sealey, W. M., Bradshaw, D., Meesala, K.-M., & Bouchard, D. A. (2024). Effects of fishmeal substitution with mealworm meals (Tenebrio molitor and Alphitobius diaperinus) on the growth, physiobiochemical response, digesta microbiome, and immune genes expression of Atlantic salmon (Salmo salar). Aquaculture Nutrition, 2024(1), 6618117.
Hall, G. M. (2010). Fishmeal production and sustainability. Fish Processing: Sustainability and New Opportunities, 207-235.
Hameed, A., Majeed, W., Naveed, M., Ramzan, U., Bordiga, M., Hameed, M., Ur Rehman, S., & Rana, N. (2022). Success of aquaculture industry with new insights of using insects as feed: a review. Fishes, 7(6), 395.
Hong, J., Han, T., & Kim, Y. Y. (2020). Mealworm (Tenebrio molitor Larvae) as an alternative protein source for monogastric animal: A review. Animals, 10(11), 2068.
Hua, K. (2021). A meta-analysis of the effects of replacing fish meals with insect meals on growth performance of fish. Aquaculture, 530, 735732.
Iaconisi, V., Secci, G., Sabatino, G., Piccolo, G., Gasco, L., Papini, A. M., & Parisi, G. (2019). Effect of mealworm (Tenebrio molitor L.) larvae meal on amino acid composition of gilthead sea bream (Sparus aurata L.) and rainbow trout (Oncorhynchus mykiss W.) fillets. Aquaculture, 513, 734403.
Khalili Tilami, S., & Sampels, S. (2018). Nutritional value of fish: lipids, proteins, vitamins, and minerals. Reviews in Fisheries Science & Aquaculture, 26(2), 243-253.
Kolawole, A., & Mustapha, A. (2023). Farmed fish feeds: use of non-conventional feed as fishmeal replacement in Nigeria. Journal of Applied Sciences and Environmental Management, 27(7), 1387-1398.
Kröncke, N., & Benning, R. (2023). Influence of dietary protein content on the nutritional composition of mealworm larvae (Tenebrio molitor L.). Insects, 14(3), 261.
La'Toya, V. L., Toddes, B. D., Wyre, N. R., Brown, D. C., Michel, K. E., & Briscoe, J. A. (2017). Effects of various diets on the calcium and phosphorus composition of mealworms (Tenebrio molitor larvae) and superworms (Zophobas morio larvae). American journal of veterinary research, 78(2), 178-185.
Makkar, H. (2018). Feed demand landscape and implications of food-not feed strategy for food security and climate change. Animal, 12(8), 1744-1754.
Mmanda, F. P. (2025). Importance of Minerals and Their Bioavailability in Boosting Aquaculture: A Systematic Review. Aquaculture, Fish and Fisheries, 5(3), e70067.
Moruzzo, R., Riccioli, F., Espinosa Diaz, S., Secci, C., Poli, G., & Mancini, S. (2021). Mealworm (Tenebrio molitor): potential and challenges to promote circular economy. Animals, 11(9), 2568.
Musharraf, M., & Khan, M. A. (2020). Dietary calcium requirement of fingerling Indian major carp, Labeo rohita (Hamilton) based on growth performance, tissue mineralization, whole body, and serum biochemical composition. Aquaculture International, 28, 1125-1139.
Quang Tran, H., Van Doan, H., & Stejskal, V. (2022). Environmental consequences of using insect meal as an ingredient in aquafeeds: A systematic view. Reviews in Aquaculture, 14(1), 237-251.
Ravzanaadii, N., Kim, S.-H., Choi, W.-H., Hong, S.-J., & Kim, N.-J. (2012). Nutritional value of mealworm, Tenebrio molitor as food source. International Journal of Industrial Entomology and Biomaterials, 25(1), 93-98.
Sankian, Z., Khosravi, S., Kim, Y.-O., & Lee, S.-M. (2018). Effects of dietary inclusion of yellow mealworm (Tenebrio molitor) meal on growth performance, feed utilization, body composition, plasma biochemical indices, selected immune parameters and antioxidant enzyme activities of mandarin fish (Siniperca scherzeri) juveniles. Aquaculture, 496, 79-87.
Serra, V., Pastorelli, G., Tedesco, D. E. A., Turin, L., & Guerrini, A. (2024). Alternative protein sources in aquafeed: Current scenario and future perspectives. Veterinary and Animal Science, 100381.
Shafique, L., Abdel-Latif, H. M., Hassan, F.-u., Alagawany, M., Naiel, M. A., Dawood, M. A., Yilmaz, S., & Liu, Q. (2021). The feasibility of using yellow mealworms (Tenebrio molitor): Towards a sustainable aquafeed industry. Animals, 11(3), 811.
Siddiqui, S. A., Elsheikh, W., Ucak, İ., Hasan, M., Perlita, Z. C., & Yudhistira, B. (2024). Replacement of soy by mealworms for livestock feed-A comparative review between soy and mealworms considering environmental aspects. Environment, Development and Sustainability, 1-44.
Siddiqui, S. A., Kamran, F., Stankov, S., Rathod, N. B., Teixeira-Costa, B. E., Fidan, H., Bhat, M. I., & Sofi, S. A. (2025). Unveiling the diversity of Non conventional Proteins-From sources, extraction, technofunctionality, nutraceutical potential to advancement in food Applications-A systematic review. Waste and Biomass Valorization, 16(1), 29-51.
Spranghers, T., Moradei, A., Vynckier, K., Boudrez, M., Pinotti, L., & Ottoboni, M. (2024). Amino acid requirements of yellow mealworm and black soldier fly larvae. Journal of Insects as Food and Feed, 1, 1-12.
Syahrulawal, L., Torske, M. O., Sapkota, R., Næss, G., & Khanal, P. (2023). Improving the nutritional values of yellow mealworm Tenebrio molitor (Coleoptera: Tenebrionidae) larvae as an animal feed ingredient: a review. Journal of Animal Science and Biotechnology, 14(1), 146.
Sadaf, T., Javid, A., Hussain, A., Bukhari, S.M., Hussain, S.M., Ain, Q., Ashraf, S., Suleman, S., Saleem, M., Azam, S.M. and Ahmad, U., 2021. Studies on parasitic prevalence in pet birds from Punjab, Pakistan. Brazilian Journal of Biology, 83, p.e246229.
Noor, R., Javid, A., Hussain, A., Bukhari, S.M., Hussain, I., Suleman, S., Malik, S., Amin, F., Azam, S.M., Ali, K. and Mustafa, G., 2021. Prevalence of parasites in selected captive bird species. Brazilian Journal of Biology, 84, p.e254251.
Hussain, M., Ali, W., Meyer, C. F. J., Javid, A., & Imran, M. (2025). Roost site selection and seasonal dynamics of the Indian flying fox (Pteropus medius): Influence of environmental and anthropogenic factors. Biosystems Diversity, 33(3), e2546-e2546.
Hussain, M., Ali, W., Meyer, C. F. J., Javid, A., & Imran, M. (2025). Characterization and metagenomics analysis of the oral microbiome of Pteropus medius: Insights from next-generation sequencing. Regulatory Mechanisms in Biosystems, 16(1), e25016-e25016.
Khan, N., Ashraf, M., Mughal, M.S., Qureshi, N.A., Khan, M.N., Rasool, F., Hafeez-ur-Rehman, M., Nasir, M., Ali, W. and Iqbal, K.J., 2014. Survival and growth potential of genetically male tilapia (GMT) fry in flow through system under different dietary protein concentrations. Pakistan Journal of Zoology, 46(2).
Khalid, N., Bukhari, S. M., Ali, W., & Sheikh, A. A. (2023). Comparative study on the predominance of Lactobacillus spp. And Escherichia coli in healthy vs colibacillosis diseased broilers. Brazilian Journal of Poultry Science, 25(03), eRBCA-2022.
Masood, S., Hussain, A., Javid, A., Bukahri, S. M., Ali, W., Ali, S., & Sattar, S. (2021). Fungal decomposition of chicken-feather waste in submerged and solid-state fermentation. Brazilian Journal of Biology, 83, e246389.
Ali, W., Javid, A., Hussain, A., & Bukhari, S. M. (2018). Diversity and conservation of freshwater turtles in Pakistan: a review. Biodiversity, 19(1-2), 62-71.
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